Wet Lab Protocols
Chromatin immunoprecipitation with spike-in calibration for quantitative comparison of protein occupancy across conditions in S. cerevisiae.
Surface-spread preparation of meiotic chromosomes for immunofluorescence microscopy of axis and cohesin proteins.
Protocol for detecting protein-protein interactions between cohesin subunits and chromosome-axis factors.
Bismaleimidoethane (BMOE) crosslinking to detect in vivo cohesin ring topology and subunit proximity.
Computational Workflows
Complete guide to installing R, RStudio, and Bioconductor packages for ChIP-seq downstream analysis on macOS.
Setting up Miniforge and bioinformatics tools (FastQC, Bowtie2, MACS3, deeptools, etc.) on an HPC cluster.
End-to-end ChIP-seq workflow: QC → trimming → alignment → peak calling → annotation → differential binding → visualisation.